RESEARCH HISTOLOGY CORE LABORATORY

The Stony Brook Research Histology Core Lab provides clinical and basic science investigators with access to a wide range of histological procedures. The facility is managed by the Department of Pathology and offers gross processing of research tissue and cellular specimens, including fixation, paraffin embedding, sectioning and staining. Both routine hematoxylin and eosin (H&E) and advanced immunohistochemical staining methods are offered. The Core staff and scientific directors are also available to assist in the development research protocols that depend on the processing of tissue or other cellular specimens. The core is directed by Dr. Kenneth Shroyer and co-directed by Dr. Jingfang Ju. Technical support is provided by Mallory Korman and Stephanie Burke.

Kenneth R. Shroyer, MD, PhD, Director of the Research Histology Core Lab, also serves as Chair of the Department of Pathology. He is a board certified surgical pathologist and cytopathologist and has been engaged in the development of advanced methods in immunohistochemistry, in situ hybridization, and the molecular analysis of tissues for over 30 years. In addition, he is an expert in tissue biobanking, microdissection, and in the use of tissue microarrays.

Mallory Korman earned her undergraduate degree in Molecular and Cellular Developmental Biology. She has had extensive training in the field of translational research histology and has been an active member of the Pathology Translational Research Laboratory in the Pathology Department since 2009. She is an experienced research histology technician with proficiency in histologic processing and sectioning of tissues, immunohistochemistry, and in the construction of tissue microarrays.   
The laboratory is equipped with automated tissue processors, an embedding station, microtomes, and automated immunostainers. Additional advanced services, including preparation of research tissue microarrays, laser capture microdissection, and other methods to support tissue-based research may be arranged following consultation with the Core Lab Director and/or the Associate Director. Please  contact the Core Lab in advance of submitting specimens to discuss methods for sample collection and tissue fixation.
  • Automated paraffin embedding
  • Histologic sectioning (paraffin sections)
  • H&E staining
  • Indirect immunoperoxidase staining
    • Protocol optimization*
    • Routine staining (manual staining)
    • Routine staining (automated immunohistochemistry)
    *Required for antibodies that are not already on line in the Research Histology Core and for any antibodies that have not been previously optimized for immunohistochemical staining on FFPE sections.

SERVICE INTERNAL EXTERNAL (minimum)
Gross prep and processing
 $17 per 5 samples ($8 each addt'l)
 $20 per 5 samples ($8 each addt'l)
Sectioning Samples by Microtome $4 per section ($2 each addt'l) $5 per section ($2 each addt'l)
Hematoxylin and Eosin Staining $4 per slide $5 per slide
Hematoxylin and Eosin Staining, Frozen
$8.00 per slide$9.00 per slide
Cryo-sectioning$8.00 per sample ($6 each addt'l)$9.00 per sample ($7 each addt'l)
Rapid Decalcification$5.00 per sample$9.00 per sample
Immunohistochemistry Initial Workup $486 per antibody $572 per antibody
Immunohistochemistry Standard $35 per slide $41 per slide
Immunohistochemistry Manual $50 per slide $59 per slide
For further information contact:
Sonya Lorrain
Basic Science Tower, Level 9
Stony Brook, NY 11794-8691 
(631) 444-3156
 sonya.lorrain@stonybrookmedicine.edu

Publication Acknowledgement:
Please acknowledge the support of the Research Histology Core Laboratory in scientific publications and provide a copy of published reports to  sonya.lorrain@stonybrookmedicine.edu.