Stony Brook University | School of Medicine | Health Sciences Center
Emily Chen, Ph.D., Assistant Professor (Pharmacology).
Emily Chen, Ph.D.
Assistant Professor
Departments of Pharmacology & Pathology
Proteomics Core Laboratory
Stony Brook Univeristy Medical Center
Stony Brook, NY 11794-8651
Tel: (631) 444-3134
Email: emily@pharm.stonybrook.edu
Research Summary:
Dr. Chen is specialized in using a mass spectrometry-based proteomic
approach to analyze complex protein mixtures. Her primary interest is to
apply her proteomic expertise in identifying determinants of
organ-specific breast cancer metastasis. Using the experimental
metastasis model, she found changes in the tumorgenecity of the cancer
cells that successfully proliferate at sites of metastasis.
Specifically, the genes and proteins expressed in those cells differ in
part from those expressed by primary tumor cells. Moreover, the changes
in expression differ accordingly to the individual metastatic site.
These data indicate that the tumor cells are selected for or adapt to
the new environments by initiating a unique program of transcriptional
and translational regulation.
Education:
| 1994-1997 | B.S. Biology, University of California, San Diego |
| 1997-2002 | Ph.D Molecular Pathology, University of California, San Diego |
Postdoctoral Training:
| 2002-2003 | Postdoctoral fellow in Dr. Carlos F. Barbas III’s Lab at the Scripps Research Institute, La Jolla, California |
| 2003-2004 | Postdoctoral fellow in Dr. Felding-Habermann’s Lab at the Scripps Research Institute, La Jolla, California |
| 2004-2007 |
Postdoctoral fellow in Dr. John R. Yates III’s Lab at the Scripps Research Institute, La Jolla, California |
Awards and Honors:
| 1997-1998 | Fellowship from the Graduate Program of Molecular Pathology at UCSD School of Medicine |
| 1998-2002 | Pre-doctoral U.S. Army Breast Cancer Fellowship |
| 2003 | Clinical Research Feasibility Funding Award (CreFF) |
| 2003-2004 | NIH post-doctoral training Fellowship |
| 2004 | AACR-WICR Brigid G. Leventhal Scholar Award |
| 2005 |
Susan G. Komen Breast Cancer Foundation Travel Award |
Publications:
Chen E.I., Yates JR 3rd, Cancer Proteomics by Quantitative Shotgun Proteomics, (2007) Molecular Oncology, 1:144-159.
Chen E.I., Cociorva D., Norris J.L., Yates JR 3rd, Optimization of Mass Spectrometry Compatible Surfactants for Shotgun Proteomics, (2007) J of Proteome Res., Jul;6(7):2529-38.
Chen E.I., Hewel J, Krueger JS, Weber MR., Tiraby C, Kralli A, Becker K, Yates JR 3rd, Felding-Habermann B, Adaptation of Energy Metabolism in Breast Cancer Brain Metastasis, (2007) Cancer Research, Feb 15;67(4):1472-86.
Chen E.I. and Yates JR 3rd. Maspin and Tumor Metastasis, (2006) IUBMB Life, Jan; 58(1):25-9.
Chen E.I., Hewel J, Felding-Habermann B., Yates JR 3rd. Large Scale Protein Profiling by Combination of Protein Fractionation and MudPIT, (2006) Molecular & Cellular Proteomics, Jan; 5(1):53-6.
Blancafort P, Chen E.I., Gonzalez B, Bergquist S, Zijlstra A, Guthy D, Brachat A, Brakenhoff R.H, Quigley JP, Erdmann D, and Barbas CF 3rd. Genetic Reprogramming of tumor cells by zinc finger transcription factors, (2005) Proc Natl Acad Sci USA 102(33): 11716-21.
Chen E.I., Florens L, Axelrod F.T., Monosov E, Barbas CF 3rd, Yates J.R. III, Felding-Habermann B, Smith J.W. Maspin alters carcinoma proteome, (2005) The FASEB Journal, published online April 27, 2005.
Radislav Sedlacek and Emily Chen. Screening for protease substrate by polyvalent phage display, (2005) Combinatorial Chemistry & High Throughput Screening, special issue: Screening for proteins & inhibitors, 8: 197-203.
Emily I. Chen, Weizhong Li, Adam Godzik, Jeffrey W. Smith. A residue in the S2 subsite controls substrate selectivity of matrix metalloproteinase-2 and matrix metalloproteinase-9, (2003) J Bio Chem 278(19): 17158-17163.
Emily I. Chen, Steven J. Kridel, Eric W. Howard, Weizhon Li, Adam Godzik and Jeffrey W. Smith. A Unique Substrate Recognition Profile for Matrix Metalloproteinase-2, (2002) J Biol Chem. 277(6): 4485-4491.
Steven J. Kridel, Hisako Sawai, Boris Ratnikov, Emily I. Chen, Weizhong Li, Adam Godzik, Alex Strongin and Jeffrey W. Smith. A Unique Substrate Binding Mode Discriminates Membrane Type-1 Matrix Metalloproteinase from Other Matrix Metalloproteinases, (2002) J Bio Chem 277(26): 23788-13793.
Steven J. Kridel, Emily Chen, Lakshmi P. Kotra, Eric W. Howard, Shahriar Mobashery, and Jeffrey W. Smith. Substrate Hydrolysis by Matrix Metalloproteinase-9, (2001) J Biol Chem. 276(23): 20572-20578.
Steven J. Kridel, Emily Chen, and Jeffrey Smith. A Substrate Phage Enzyme-Linked Immunosorbent Assay to Profile Panels of Proteases, (2001) Analytical Biochemistry. 294: 176-184.
Henry C. Powell, Robert S. Garrett, Francesca M Brett, Chi-Shuin Chiang, Emily Chen, Eliezer Masliah and Iain L. Campbell. Response of Glia, Mast Cells and the Blood Brain Barrier, in Transgenic Mice Expressing Interleukin-3 in Astrocytes, an Experimental Model for CNS Demyelination, (1999) Brain Pathology. 9: 219-235.
